【佳學(xué)基因檢測】miR-205在前列腺癌中的表達(dá)及與患者預(yù)后的關(guān)系

國內(nèi)腫瘤基因檢測權(quán)威機(jī)構(gòu)名單解密

體會聽到《Comput Math Methods Med》在.?2022 Aug 30;2022:1784791.發(fā)表了一篇題目為《miR-205在前列腺癌中的表達(dá)及與患者預(yù)后的關(guān)系》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Zhuifeng Guo,?Xuwei Lu,?Fan Yang,?Liang Qin,?Ning Yang,?Peiran Cai,?Conghui Han,?Jiawen Wu,?Hang Wang?等完成。促進(jìn)了腫瘤的正確治療與個(gè)性化用藥的發(fā)展，進(jìn)一步強(qiáng)調(diào)了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內(nèi)容關(guān)鍵詞：

腫瘤靶向治療基因檢測臨床應(yīng)用結(jié)果

目的：我們旨在探討前列腺癌患者血清和細(xì)胞外泌體miR-205水平的變化及其臨床意義。材料與方法：首先，利用UALCAN數(shù)據(jù)庫分析miR-205在正常和前列腺癌組織中的表達(dá)。 .分析腫瘤組織中miR-205與前列腺癌患者病理及臨床特征的關(guān)系。因此，于2016年8月至2021年8月在閔行醫(yī)院收集60例前列腺癌患者。采集兩組患者的血清，提取血清外泌體中的RNA，采用qRT-PCR分析miR的表達(dá)。 -205 由血清外泌體介導(dǎo)。同時(shí)，比較了前列腺癌患者的臨床及病理特征與外泌體介導(dǎo)的miR-205表達(dá)水平之間的關(guān)系。接下來，使用傷口愈合和 CKK-8 等檢測方法研究 miR-205 在從前列腺癌中提取的外泌體中對前列腺癌增大和轉(zhuǎn)移的影響。結(jié)果：結(jié)果表明 miR-205 在組織中的顯著水平前列腺癌明顯低于正常前列腺組織。此外，miR-205在前列腺癌患者的液體外泌體和來自前列腺癌細(xì)胞系的外泌體中的表達(dá)水平明顯低于健康患者的血清外泌體和前列腺正常細(xì)胞系中的表達(dá)水平。前列腺癌患者流體外泌體中 miR-205 的表達(dá)水平與癌癥分期、淋巴結(jié)中不受控制的細(xì)胞分裂、遠(yuǎn)處轉(zhuǎn)移和初始診斷時(shí)的 PSA 水平呈負(fù)相關(guān)。分析（多變量和單變量）表明，miR-205 的表達(dá)是前列腺癌患者預(yù)后的主權(quán)威脅原因。此外，miR-205的過表達(dá)可以限制前列腺癌的表達(dá)和轉(zhuǎn)移。結(jié)論：液體來源的外泌體中miR-205的表達(dá)與前列腺癌患者的預(yù)測相關(guān)，可能是一種新的鑒定標(biāo)志物。和治療前列腺癌。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國際數(shù)據(jù)庫描述：

Purpose:?We aimed to investigate the changes of serum and cell exosome miR-205 levels in patients with prostate carcinoma and its clinical significance.Materials and methods:?Firstly, pronouncement of miR-205 in normal and prostate carcinoma tissues was analyzed by using UALCAN database. The relationship between miR-205 in tumor tissues and the pathological and clinical characteristics of patients with prostate carcinoma were analyzed. Consequently, 60 people with prostate carcinoma were collected to the Minhang Hospital from August 2016 to August 2021. Serum of patients in the two groups was collected, and RNA in serum exosomes was extracted, and qRT-PCR was used to analyze the expression of miR-205 mediated by serum exosomes. Meanwhile, the relationship among the clinical as well as pathological aspects and bodement of patients with prostate carcinoma and the pronouncement level of miR-205 mediated by exosome was compared. Next, assays like wound healing and CKK-8 were used to investigate the effects of miR-205 in exosomes extracted from prostate carcinoma on the augmentation and metastasis of prostate carcinoma.Results:?The results showed that the pronouncement level of miR-205 in tissues with prostate carcinoma was significantly lower than that in normal prostate tissues. In addition, the pronouncement level of miR-205 in fluid exosome of people with prostate carcinoma and exosomes derived from the lines of prostate carcinoma was considerably less than that in serum exosomes of healthy patients and that of normal cell lines of prostate. The pronouncement level of miR-205 in fluid exosomes of people with prostate carcinoma was negatively associated with cancer phase, uncontrolled cell division in lymph nodes, distant metastasis, and PSA level at initial diagnosis. Analysis (multivariate and univariate) showed that miR-205 pronouncement was a sovereign threat cause for prognosis of prostate cancer patients. Additionally, the pronouncement and metastasis of prostate carcinoma can be restricted by the overexpression of miR-205.Conclusion:?The pronouncement of miR-205 in liquid derived exosomes is correlated with the prediction of people with prostate carcinoma and may be a new marker for identification and cure of prostate carcinoma.