【佳學(xué)基因檢測】MicroRNA-18a 通過HIF-1α 表達(dá)調(diào)節(jié)口腔鱗狀細(xì)胞癌細(xì)胞的轉(zhuǎn)移特性

品牌基因檢測在哪里做——時(shí)機(jī)窗口

開題評估知道《BMC Oral Health》在. 2022 Sep 5;22(1):378.發(fā)表了一篇題目為《MicroRNA-18a 通過 HIF-1α 表達(dá)調(diào)節(jié)口腔鱗狀細(xì)胞癌細(xì)胞的轉(zhuǎn)移特性》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Shihyun Kim, Suyeon Park, Ji-Hyeon Oh, Sang Shin Lee, Yoon Lee, Jongho Choi 等完成。促進(jìn)了腫瘤的正確治療與個(gè)性化用藥的發(fā)展，進(jìn)一步強(qiáng)調(diào)了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內(nèi)容關(guān)鍵詞：

HIF-1α,缺氧,入侵,微小RNA,移民,口腔鱗狀細(xì)胞癌

腫瘤靶向治療基因檢測臨床應(yīng)用結(jié)果

口腔鱗狀細(xì)胞癌轉(zhuǎn)移風(fēng)險(xiǎn)與靶向藥物治療基因檢測背景：口腔鱗狀細(xì)胞癌的快速轉(zhuǎn)移與預(yù)后不良和高死亡率相關(guān)。然而，總口腔鱗狀細(xì)胞癌轉(zhuǎn)移的分子機(jī)制尚未有效闡明。盡管 microRNA (miRNA) 的失調(diào)表達(dá)在惡性腫瘤進(jìn)展中具有關(guān)鍵作用，但 miRNA 在 總口腔鱗狀細(xì)胞癌進(jìn)展中的生物學(xué)功能仍不清楚。口腔鱗狀細(xì)胞癌轉(zhuǎn)移風(fēng)險(xiǎn)與靶向藥物治療基因檢測研究旨在探討 miRNA-18a 通過缺氧誘導(dǎo)因子 1α (HIF-1α) 在 總口腔鱗狀細(xì)胞癌轉(zhuǎn)移調(diào)控中的作用。口腔鱗狀細(xì)胞癌轉(zhuǎn)移風(fēng)險(xiǎn)與靶向藥物治療基因檢測方法： miRNA-18a-5p (miRNA-18a) 在 總患者 (n = 39) 和在 總口腔鱗狀細(xì)胞癌細(xì)胞系（例如，YD-10B 和 HSC-2 細(xì)胞）中，使用定量實(shí)時(shí)聚合酶鏈反應(yīng)進(jìn)行分析。使用蛋白質(zhì)印跡分析用 miRNA-18a 模擬物或與氯化鈷組合處理的 總口腔鱗狀細(xì)胞癌細(xì)胞中的 HIF-1α 蛋白表達(dá)。采用MTT法、EdU法和Transwell®插入系統(tǒng)分析了總生存期CC細(xì)胞的miRNA-18a表達(dá)依賴性增殖和侵襲能力。口腔鱗狀細(xì)胞癌轉(zhuǎn)移風(fēng)險(xiǎn)與靶向藥物治療基因檢測結(jié)果：總生存期CC組織中miRNA-18a表達(dá)明顯低于鄰近正常組織。在 總口腔鱗狀細(xì)胞癌細(xì)胞系中，HIF-1α 表達(dá)通過 miRNA-18a 模擬處理顯著降低。此外，與僅用 miRNA-18a 模擬物處理的細(xì)胞相比，miRNA-18a 模擬物顯著降低 總口腔鱗狀細(xì)胞癌細(xì)胞的遷移和侵襲能力，而在缺氧條件下 HIF-1α 的過表達(dá)顯著提高了 總口腔鱗狀細(xì)胞癌細(xì)胞的遷移和侵襲能力?？谇击[狀細(xì)胞癌轉(zhuǎn)移風(fēng)險(xiǎn)與靶向藥物治療基因檢測結(jié)論：miRNA-18a對 HIF-1α 表達(dá)產(chǎn)生負(fù)面影響并抑制 總口腔鱗狀細(xì)胞癌的轉(zhuǎn)移，從而表明其作為 總口腔鱗狀細(xì)胞癌抗轉(zhuǎn)移策略的治療靶點(diǎn)的潛力。缺氧;入侵；微小RNA；移民;口腔鱗狀細(xì)胞癌。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國際數(shù)據(jù)庫描述：

Background: Rapid metastasis of oral squamous cell carcinoma (OSCC) is associated with a poor prognosis and a high mortality rate. However, the molecular mechanisms underlying OSCC metastasis have not been fully elucidated. Although deregulated expression of microRNA (miRNA) has a crucial role in malignant cancer progression, the biological function of miRNA in OSCC progression remains unclear. This study aimed to investigate the function of miRNA-18a in OSCC metastatic regulation via hypoxia-inducible factor 1α (HIF-1α).Methods: miRNA-18a-5p (miRNA-18a) expressions in patients with OSCC (n = 39) and in OSCC cell lines (e.g., YD-10B and HSC-2 cells) were analyzed using quantitative real-time polymerase chain reaction. HIF-1α protein expressions in OSCC cells treated with miRNA-18a mimics or combined with cobalt chloride were analyzed using western blotting. The miRNA-18a expression-dependent proliferation and invasion abilities of OSCC cells were analyzed using MTT assay, EdU assay, and a Transwell® insert system.Results: miRNA-18a expression was significantly lower in OSCC tissue than in the adjacent normal tissue. In OSCC cell lines, HIF-1α expression was significantly decreased by miRNA-18a mimic treatment. Furthermore, the migration and invasion abilities of OSCC cells were significantly decreased by miRNA-18a mimics and significantly increased by the overexpression of HIF-1α under hypoxic conditions relative to those abilities in cells treated only with miRNA-18a mimics.Conclusions: miRNA-18a negatively affects HIF-1α expression and inhibits the metastasis of OSCC, thereby suggesting its potential as a therapeutic target for antimetastatic strategies in OSCC.Keywords: HIF-1α; Hypoxia; Invasion; MicroRNA; Migration; Oral squamous cell carcinoma.

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